Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10389862" target="_blank" >RIV/00216208:11310/18:10389862 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s00709-018-1225-1" target="_blank" >https://doi.org/10.1007/s00709-018-1225-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-018-1225-1" target="_blank" >10.1007/s00709-018-1225-1</a>

Result language

angličtina

Original language name

Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

Original language description

Species of Zygnema form macroscopically visible mats in polar and temperate terrestrial habitats, where they are exposed to environmental stresses. Three previously characterized isolates (Arctic Zygnema sp. B, Antarctic Zygnema sp. C, and temperate Zygnema sp. S) were tested for their tolerance to experimental UV radiation. Samples of young vegetative cells (1 month old) and pre-akinetes (6 months old) were exposed to photosynthetically active radiation (PAR, 400-700 nm, 400 mu mol photons m(-2) s(-1)) in combination with experimental UV-A (315-400 nm, 5.7 W m(-2), no UV-B), designated as PA, or UV-A (10.1 W m(-2)) + UV-B (280-315 nm, 1.0 W m(-2)), designated as PAB. The experimental period lasted for 74 h; the radiation period was 16 h PAR/UV-A per day, or with additional UV-B for 14 h per day. The effective quantum yield, generally lower in pre-akinetes, was mostly reduced during the UV treatment, and recovery was significantly higher in young vegetative cells vs. pre-akinetes during the experiment. Analysis of the deepoxidation state of the xanthophyll-cycle pigments revealed a statistically significant (p &lt; 0.05) increase in Zygnema spp. C and S. The content of UV-absorbing phenolic compounds was significantly higher (p &lt; 0.05) in young vegetative cells compared to pre-akinetes. In young vegetative Zygnema sp. S, these phenolic compounds significantly increased (p &lt; 0.05) upon PA and PAB. Transmission electron microscopy showed an intact ultrastructure with massive starch accumulations at the pyrenoids under PA and PAB. A possible increase in electron-dense bodies in PAB-treated cells and the occurrence of cubic membranes in the chloroplasts are likely protection strategies. Metabolite profiling by non-targeted RP-UHPLC-qToF-MS allowed a clear separation of the strains, but could not detect changes due to the PA and PAB treatments. Six hundred seventeen distinct molecular masses were detected, of which around 200 could be annotated from databases. These results indicate that young vegetative cells can adapt better to the experimental UV-B stress than pre-akinetes.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/GF15-34645L" target="_blank" >GF15-34645L: Colonization of Land by Conjugating Green Algae</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protoplasma

ISSN

0033-183X

e-ISSN

—

Volume of the periodical

255

Issue of the periodical within the volume

4

Country of publishing house

AT - AUSTRIA

Number of pages

14

Pages from-to

1239-1252

UT code for WoS article

000434795100021

EID of the result in the Scopus database

2-s2.0-85042388098