DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10393720" target="_blank" >RIV/00216208:11310/18:10393720 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/18:00503521 RIV/61388963:_____/18:00499541

Result on the web

<a href="http://www.biochemj.org/content/475/23/3847" target="_blank" >http://www.biochemj.org/content/475/23/3847</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/BCJ20180764" target="_blank" >10.1042/BCJ20180764</a>

Result language

angličtina

Original language name

DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors

Original language description

Influenza neuraminidase is responsible for the escape of new viral particles from the infected cell surface. Several neuraminidase inhibitors are used clinically to treat patients or stockpiled for emergencies. However, the increasing development of viral resistance against approved inhibitors has underscored the need for the development of new antivirals effective against resistant influenza strains. A facile, sensitive, and inexpensive screening method would help achieve this goal. Recently, we described a multiwell plate-based DNA-linked inhibitor antibody assay (DIANA). This highly sensitive method can quantify femtomolar concentrations of enzymes. DIANA also has been applied to high-throughput enzyme inhibitor screening, allowing the evaluation of inhibition constants from a single inhibitor concentration. Here, we report the design, synthesis, and structural characterization of a tamiphosphor derivative linked to a reporter DNA oligonucleotide for the development of a DIANA-type assay to screen potential influenza neuraminidase inhibitors. The neuraminidase is first captured by an immobilized antibody, and the test compound competes for binding to the enzyme with the oligo-linked detection probe, which is then quantified by qPCR. We validated this novel assay by comparing it with the standard fluorometric assay and demonstrated its usefulness for sensitive neuraminidase detection as well as high-throughput screening of potential new neuraminidase inhibitors.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LO1302" target="_blank" >LO1302: InterBioMed</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Journal

ISSN

0264-6021

e-ISSN

—

Volume of the periodical

475

Issue of the periodical within the volume

23

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

3847-3860

UT code for WoS article

000463667300002

EID of the result in the Scopus database

2-s2.0-85058399587