Inkjet-Printed Carbon Nanotube Electrodes for Measuring Pyocyanin and Uric Acid in a Wound Fluid Simulant and Culture Media
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10396642" target="_blank" >RIV/00216208:11310/19:10396642 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qzae8MqqmE" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qzae8MqqmE</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.8b05591" target="_blank" >10.1021/acs.analchem.8b05591</a>
Alternative languages
Result language
angličtina
Original language name
Inkjet-Printed Carbon Nanotube Electrodes for Measuring Pyocyanin and Uric Acid in a Wound Fluid Simulant and Culture Media
Original language description
Polyacrylamide-coated, carbon nanotube (PA/CNT) electrodes were prepared by an inkjet printing process and used to measure pyocyanin and uric acid in a wound fluid simulant at 37 degrees C. These two molecules are potential indicators of infection, and therefore their detection could prove useful for monitoring wound healing. Pyocyanin is a marker for the common wound bacterium Pseudomonas aeruginosa. Our long-term goal is to use these inexpensive and disposable electrodes to measure biomarkers of wound healing directly. In this proof-of-concept work, studies were performed in a wound fluid simulant to evaluate the stability of the electrodes and their responsiveness for the two bioanalytes. The PA/CNT inkjet-printed electrodes and electrical contacts were stable with unchanging physical and electrochemical properties in the wound fluid simulant over a 7-8-day period at 37 degrees C. The detection figures of merit for pyocyanin in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 0.10 to 100 mu mol L-1 (R-2 = 0.9992), limit of detection = 0.10 mu mol L-1 (S/N = 3), sensitivity = 35.6 +/- 0.8 mA-L mol(-1) and response variability <= 4% RSD. The detection figures of merit for uric acid in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 100 to 1000 mu mol L-1 (R-2 = 0.9997), sensitivity = 2.83 +/- 0.01 mA-L mol(-1), and response variability <= 4% RSD. The limit of detection was not determined. The PA/CNT electrodes were also used to quantify pyocyanin concentrations in cell-free culture media from different strains of P. aeruginosa. The detected concentrations ranged from 1.00 +/- 0.02 to 118 +/- 6 mu M depending on the strain.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
91
Issue of the periodical within the volume
14
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
8835-8844
UT code for WoS article
000476567700019
EID of the result in the Scopus database
2-s2.0-85069949132