Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10403675" target="_blank" >RIV/00216208:11310/19:10403675 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=MH4CKxocfk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=MH4CKxocfk</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2019.104137" target="_blank" >10.1016/j.microc.2019.104137</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study
Original language description
A series of biologically important 7-deazapurine nucleosides was used for evaluation of selectivity of three stationary phases designed for supercritical fluid chromatography. The sorbents of stationary phases tested were diethylamine, diol and 2-picolylamine bonded on ethylene bridged hybrid particles. The highest selectivity for the tested nucleosides showed neutral diol column. All twelve tested nucleosides were baseline resolved in simple mobile phase composed of carbon dioxide/ethanol 79/21 (v/v). The effect of basic isopropylamine and mixed isopropylamine and trifluoroacetic acid mobile phase additives on retention and selectivity was also assessed. The interactions participating in the separation systems were revealed by linear free energy relationship. The obtained results demonstrated the significant contribution of hydrogen bond acidity and basicity on chromatographic behavior of the diol- and 2-picolylamine-based stationary phases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
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Volume of the periodical
150
Issue of the periodical within the volume
November
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
104137
UT code for WoS article
000495469400060
EID of the result in the Scopus database
2-s2.0-85069898772