Establishment and characterization of a mouse tumor cell line with irreversible downregulation of MHC class I molecules

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10404119" target="_blank" >RIV/00216208:11310/19:10404119 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3XMmLHE-Jy" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3XMmLHE-Jy</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2019.7356" target="_blank" >10.3892/or.2019.7356</a>

Result language

angličtina

Original language name

Establishment and characterization of a mouse tumor cell line with irreversible downregulation of MHC class I molecules

Original language description

In the majority of human tumors, downregulation of major histocompatibility complex class I (MHC-I) expression contributes to the escape from the host immune system and resistance to immunotherapy. Relevant animal models are therefore needed to enhance the efficacy of cancer immunotherapy. As loss of beta-2 microglobulin expression results in irreversible downregulation of surface MHC-I molecules in various human tumors, the beta-2 microglobulin gene (B2m) was deactivated in a mouse oncogenic TC-1 cell line and a TC-1/dB2m cell line that was negative for surface MHC-I expression was derived. Following stimulation with interferon gamma, MHC-I heavy chains, particularly the H-2D(b) molecules, were found to be expressed at low levels on the cell surface, but without beta-2 microglobulin. B2m deactivation in TC-1/dB2m cells led to reduced proliferation and tumor growth. These cells were insensitive to DNA vaccination and only weakly responsive to combined immunotherapy with a DNA vaccine and the ODN1826 adjuvant. In vivo depletion demonstrated that NK1.1(+) cells were involved in both reduced tumor growth and an antitumor effect of immunotherapy. The number of immune cells infiltrating TC-1/dB2m-induced tumors was comparable with that in tumors developing from TC-1/A9 cells characterized by reversible MHC-I downregulation. However, the composition of the cell infiltrate was different and, most importantly, infiltration with immune cells was not increased in TC-1/dB2m tumors after immunotherapy. Therefore, the TC-1/dB2m cell line represents a clinically relevant tumor model that may be used for enhancement of cancer immunotherapy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology Reports

ISSN

1021-335X

e-ISSN

—

Volume of the periodical

42

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

10

Pages from-to

2826-2835

UT code for WoS article

000503165000058

EID of the result in the Scopus database

2-s2.0-85074619808