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Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409344" target="_blank" >RIV/00216208:11310/19:10409344 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/19:00112932 RIV/67985904:_____/19:00537765

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BLdyGWgf_h" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BLdyGWgf_h</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/biolre/ioz117" target="_blank" >10.1093/biolre/ioz117</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

  • Original language description

    Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10605 - Developmental biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biology of Reproduction

  • ISSN

    0006-3363

  • e-ISSN

  • Volume of the periodical

    101

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    719-732

  • UT code for WoS article

    000509503400008

  • EID of the result in the Scopus database

    2-s2.0-85075813083