beta-Arrestin 2 and ERK1/2 Are Important Mediators Engaged in Close Cooperation between TRPV1 and mu-Opioid Receptors in the Plasma Membrane
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10415788" target="_blank" >RIV/00216208:11310/20:10415788 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=._o0L_sfUA" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=._o0L_sfUA</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21134626" target="_blank" >10.3390/ijms21134626</a>
Alternative languages
Result language
angličtina
Original language name
beta-Arrestin 2 and ERK1/2 Are Important Mediators Engaged in Close Cooperation between TRPV1 and mu-Opioid Receptors in the Plasma Membrane
Original language description
The interactions between TRPV1 and mu-opioid receptors (MOR) have recently attracted much attention because these two receptors play important roles in pain pathways and can apparently modulate each other's functioning. However, the knowledge about signaling interactions and crosstalk between these two receptors is still limited. In this study, we investigated the mutual interactions between MOR and TRPV1 shortly after their activation in HEK293 cells expressing these two receptors. After activation of one receptor we observed significant changes in the other receptor's lateral mobility and vice versa. However, the changes in receptor movement within the plasma membrane were not connected with activation of the other receptor. We also observed that plasma membrane beta-arrestin 2 levels were altered after treatment with agonists of both these receptors. Knockdown of beta-arrestin 2 blocked all changes in the lateral mobility of both receptors. Furthermore, we found that beta-arrestin 2 can play an important role in modulating the effectiveness of ERK1/2 phosphorylation after activation of MOR in the presence of TRPV1. These data suggest that beta-arrestin 2 and ERK1/2 are important mediators between these two receptors and their signaling pathways. Collectively, MOR and TRPV1 can mutually affect each other's behavior and beta-arrestin 2 apparently plays a key role in the bidirectional crosstalk between these two receptors in the plasma membrane.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences [online]
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
4626
UT code for WoS article
000550282100001
EID of the result in the Scopus database
2-s2.0-85087120943