The effect of particle and ligand types on retention and peak shape in liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10416654" target="_blank" >RIV/00216208:11310/20:10416654 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=z_IygrEP2v" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=z_IygrEP2v</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2020.105466" target="_blank" >10.1016/j.microc.2020.105466</a>
Alternative languages
Result language
angličtina
Original language name
The effect of particle and ligand types on retention and peak shape in liquid chromatography
Original language description
A well performed, detailed characterization of stationary phase properties can serve as a useful tool for choice of suitable separation environment for a given purpose. This work shows effects of particle type/technology and ligand chemistry on retention and peak shape of neutral, acidic, basic and zwitterionic compounds. Eight columns differing in endcapping technology, particle type and ligand bonded were evaluated, namely XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XBridge C18 and XBridge Shield RP18. A simple Walters test for evaluation of hydrophobicity and silanol activity was performed. Mobile phases composed of acetonitrile and aqueous part (pH 2.1; pH 4.7 and pH 8.0) were used. More detailed description of interactions participating in the retention mechanism was provided by the linear free energy relationship model. For a more detailed characterization of possible electrostatic interactions, compounds with permanent charge across selected pH range were used. A good agreement between the Walters test results, the expanded linear free energy relationship model, and the test of ionic-interactions was observed, suggesting that each testing method provides value for characterization of the solute-stationary phase interactions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA20-19655S" target="_blank" >GA20-19655S: Strategy for on-line protein digestion followed by separation in mixed-mode chromatography</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
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Volume of the periodical
159
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
105466
UT code for WoS article
000598759800007
EID of the result in the Scopus database
2-s2.0-85090131928