Electroanalytical methods for determination of 7-dehydrocholesterol in artificial serum

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420322" target="_blank" >RIV/00216208:11310/20:10420322 - isvavai.cz</a>
Result on the web
<a href="http://web.natur.cuni.cz/analchem/isc/isc16.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc16.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electroanalytical methods for determination of 7-dehydrocholesterol in artificial serum
Original language description
7-Dehydrocholesterol is a biomarker of Smith-Lemli-Opitz syndrome, an autosomal recessive genetic disorder, caused by the inborn deficiency of 7-dehydrocholesterol reductase. In this study, procedures for its determination in artificial serum using flow injection analysis with electrochemical detection and voltammetric detection on boron doped diamond electrode were optimized. The proteins were precipitated by acetonitrile and after centrifugation the supernatant used for analysis. For quantitation of 7-DHC by differential pulse voltammetry the optimal ratio acetonitrile-artificial serum 9:1 (v/v) was applied. In FIA-ED, the ratio 3:1 (v/v), run electrolyte consisting of water-acetonitrile containing 0.01 mol L-1 NaClO in the same ratio 4 and detection potential of +1.3 V vs. Ag/AgCl (3 mol L-1 KCl) were used. Quantitation of 7-DHC was possible using calibration depen- dence with limit detection of 20 μmol L-1 in artificial serum. Nevertheless, the method has low recovery and for sensitive determination in real matrices of human serum and amniotic fluid a liquid-liquid extraction needs to be applied to prevent presence of 7-dehydrocholesterol in the phase with precipitated proteins.
Czech name
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Czech description
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Project
<a href="/en/project/GA19-11268S" target="_blank" >GA19-11268S: Electrochemical Methods: New Approaches for Characterization and Analysis of Steroids</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 16th International Students Conference "Modern Analytical Chemistry"
ISBN
978-80-7444-079-3
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
146-150
Publisher name
Univerzita Karlova, Přírodovědecká fakulta
Place of publication
Praha
Event location
Prague
Event date
Sep 17, 2020
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000623101400025

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