AFB1 controls rapid auxin signalling through membrane depolarization in Arabidopsis thaliana root
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10432709" target="_blank" >RIV/00216208:11310/21:10432709 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22340/21:43922975 RIV/49777513:23640/21:43962550
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_AVPrO89M5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_AVPrO89M5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41477-021-00969-z" target="_blank" >10.1038/s41477-021-00969-z</a>
Alternative languages
Result language
angličtina
Original language name
AFB1 controls rapid auxin signalling through membrane depolarization in Arabidopsis thaliana root
Original language description
The membrane potential reflects the difference between cytoplasmic and apoplastic electrical potentials and is essential for cellular operation. The application of the phytohormone auxin (3-indoleacetic acid (IAA)) causes instantaneous membrane depolarization in various cell types(1-6), making depolarization a hallmark of IAA-induced rapid responses. In root hairs, depolarization requires functional IAA transport and TIR1-AFB signalling(5), but its physiological importance is not understood. Specifically in roots, auxin triggers rapid growth inhibition(7-9) (RGI), a process required for gravitropic bending. RGI is initiated by the TIR1-AFB co-receptors, with the AFB1 paralogue playing a crucial role(10,11). The nature of the underlying rapid signalling is unknown, as well as the molecular machinery executing it. Even though the growth and depolarization responses to auxin show remarkable similarities, the importance of membrane depolarization for root growth inhibition and gravitropism is unclear. Here, by combining the DISBAC(2)(3) voltage sensor with microfluidics and vertical-stage microscopy, we show that rapid auxin-induced membrane depolarization tightly correlates with RGI. Rapid depolarization and RGI require the AFB1 auxin co-receptor. Finally, AFB1 is essential for the rapid formation of the membrane depolarization gradient across the gravistimulated root. These results clarify the role of AFB1 as the central receptor for rapid auxin responses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GJ18-10116Y" target="_blank" >GJ18-10116Y: Visualization of root growth physiology and deciphering molecular mechanisms that govern root cell expansion in Arabidopsis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Plants
ISSN
2055-026X
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
1229-1238
UT code for WoS article
000674542500001
EID of the result in the Scopus database
2-s2.0-85110717687