Nuclear copies of mitochondrial DNA as a potential problem for phylogenetic and population genetic studies of Odonata

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10450325" target="_blank" >RIV/00216208:11310/22:10450325 - isvavai.cz</a>

Alternative codes found

RIV/61988987:17310/22:A2302G7Y

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=K_cYow2~Gj" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=K_cYow2~Gj</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/syen.12550" target="_blank" >10.1111/syen.12550</a>

Result language

angličtina

Original language name

Nuclear copies of mitochondrial DNA as a potential problem for phylogenetic and population genetic studies of Odonata

Original language description

The use of mitochondrial markers for taxonomic identification and biodiversity monitoring is not without risks or limitations. Most importantly, the natural transfer of DNA from the mitochondria to the nucleus generates nonfunctional nuclear copies of mitochondrial DNA (NUMTs). Their abundance and size vary significantly among taxa, and NUMTs have been reported to complicate molecular studies based on mitochondrial markers in several insect orders, most prominently in Orthoptera. The significance of this phenomenon in Odonata has not yet been properly addressed. Here, we present a complete mitochondrial genome and a draft nuclear genome of Leucorrhinia albifrons (Odonata: Libellulidae), as well as NUMT and cox1 sequences from the related species Leucorrhinia dubia. We document the presence of NUMTs in the L. albifrons nuclear genome and in nuclear genomes of two other Odonata species available in public databases. Our results show that NUMTs can have a serious impact on barcoding, phylogenetic, population and phylogeographic studies of Odonata, especially when the barcode is located in the cox1 gene, the most frequently used molecular marker for Odonata. We suggest that nad1 should be used alone or in combination with cox1 to minimize unintended confusion with NUMTs. Finally, we present a mitophylogenomic analysis of Odonata and document several cases of misidentified mitochondrial genomes belonging to species different from those indicated in public databases. In conclusion, our findings represent an important step for future metabarcoding studies of Odonata based on mitochondrial DNA markers.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10613 - Zoology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Systematic Entomology

ISSN

0307-6970

e-ISSN

1365-3113

Volume of the periodical

47

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

591-602

UT code for WoS article

000788498900001

EID of the result in the Scopus database

2-s2.0-85128916984