Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10451267" target="_blank" >RIV/00216208:11310/22:10451267 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=OGUn9kOkni" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=OGUn9kOkni</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2022.463201" target="_blank" >10.1016/j.chroma.2022.463201</a>
Alternative languages
Result language
angličtina
Original language name
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
Original language description
Ion-pairing reversed-phase liquid chromatography was utilized for the analysis of native and phosphorothioate oligonucleotides of the identical sequence but different amount and position of phosphorothioate modifications. The effects of ion-pairing amines nature (alkyl chain length, hydrophobicity) and concentration on retention, n / n-1 resolution, and diastereomeric separation of phosphorothioate oligonucleotides were investigated using octadecyl column. Eight different ion-pairing agents at two concentrations (10 mM and 100 mM) buffered with acetic acid were investigated. The resolution of n and n-1 mers oligonucleotides improved with hydrophobicity and concentration of ion-pairing amines. Ion-pairing amines with moderate hydrophobicity were most successful in suppressing diastereomeric resolution. However, a partial separation of phosphorothioate oligonucleotide diastereomers was observed with all ion-pairing systems, which resulted in wider peaks compared to phosphorodiester oligonucleotides of the same sequence. This phenomenon complicates the n and n-1 mers separation of oligonucleotides with high degree of phosphorothioate modifications. Separation of oligonucleotides with different number of phosphorothioate modifications was observed, which may be useful for therapeutic oligonucleotide analysis. The aim of the work was to identify the ion-pairing systems useful for chromatographic characterization of phosphorothioate oligonucleotides.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA20-19655S" target="_blank" >GA20-19655S: Strategy for on-line protein digestion followed by separation in mixed-mode chromatography</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
1873-3778
Volume of the periodical
1676
Issue of the periodical within the volume
August
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
463201
UT code for WoS article
000827852700010
EID of the result in the Scopus database
2-s2.0-85132740521