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Meiotic chromosome dynamics and double strand break formation in reptiles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10453745" target="_blank" >RIV/00216208:11310/22:10453745 - isvavai.cz</a>

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rSk.eV4yZW" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rSk.eV4yZW</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fcell.2022.1009776" target="_blank" >10.3389/fcell.2022.1009776</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Meiotic chromosome dynamics and double strand break formation in reptiles

  • Original language description

    During meiotic prophase I, tightly regulated processes take place, from pairing and synapsis of homologous chromosomes to recombination, which are essential for the generation of genetically variable haploid gametes. These processes have canonical meiotic features conserved across different phylogenetic groups. However, the dynamics of meiotic prophase I in non-mammalian vertebrates are poorly known. Here, we compare four species from Sauropsida to understand the regulation of meiotic prophase I in reptiles: the Australian central bearded dragon (Pogona vitticeps), two geckos (Paroedura picta and Coleonyx variegatus) and the painted turtle (Chrysemys picta). We first performed a histological characterization of the spermatogenesis process in both the bearded dragon and the painted turtle. We then analyzed prophase I dynamics, including chromosome pairing, synapsis and the formation of double strand breaks (DSBs). We show that meiosis progression is highly conserved in reptiles with telomeres clustering forming the bouquet, which we propose promotes homologous pairing and synapsis, along with facilitating the early pairing of micro-chromosomes during prophase I (i.e., early zygotene). Moreover, we detected low levels of meiotic DSB formation in all taxa. Our results provide new insights into reptile meiosis.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10613 - Zoology

Result continuities

  • Project

    <a href="/en/project/GC20-27236J" target="_blank" >GC20-27236J: Comparative and genomic perspective on karyotype evolution in reptiles</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Cell and Developmental Biology

  • ISSN

    2296-634X

  • e-ISSN

    2296-634X

  • Volume of the periodical

    10

  • Issue of the periodical within the volume

    October

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    15

  • Pages from-to

    1009776

  • UT code for WoS article

    000876737000001

  • EID of the result in the Scopus database

    2-s2.0-85140654892