RNAi-mediated gene silencing of Phlebotomus papatasi defensins favors Leishmania major infection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10465934" target="_blank" >RIV/00216208:11310/23:10465934 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Gu5i1.7fSQ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Gu5i1.7fSQ</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2023.1182141" target="_blank" >10.3389/fphys.2023.1182141</a>
Alternative languages
Result language
angličtina
Original language name
RNAi-mediated gene silencing of Phlebotomus papatasi defensins favors Leishmania major infection
Original language description
Introduction: Production of different antimicrobial peptides (AMPs) is one of the insect's prominent defense strategies, regulated mainly by Toll and immune deficiency (IMD) humoral pathways. Here we focused mainly on two AMPs of Phlebotomus papatasi, vector of Leishmania major parasites, their association with the relish transcription factor and the effective participation on Leishmania infection.Methods and results: We further characterized the role of previously described gut-specific P. papatasi defensin (PpDef1) and identified the second defensin (PpDef2) expressed in various sand fly tissues. Using the RNAi-mediated gene silencing, we report that the silencing of PpDef1 gene or simultaneous silencing of both defensin genes (PpDef1 and PpDef2) resulted in increased parasite levels in the sand fly (detectable by PCR) and higher sand fly mortality. In addition, we knocked down relish, the sole transcription factor of the IMD pathway, to evaluate the association of the IMD pathway with AMPs expression in P. papatasi. We demonstrated that the relish gene knockdown reduced the expression of PpDef2 and attacin, another AMP abundantly expressed in the sand fly body.Conclusions: Altogether, our experiments show the importance of defensins in the sand fly response toward L. major and the role of the IMD pathway in regulating AMPs in P. papatasi.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GA21-15700S" target="_blank" >GA21-15700S: Leishmania-sand fly interaction: new approaches to answer old questions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Physiology
ISSN
1664-042X
e-ISSN
1664-042X
Volume of the periodical
14
Issue of the periodical within the volume
09 May 2023
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
1182141
UT code for WoS article
001016617700001
EID of the result in the Scopus database
2-s2.0-85161009505