Fish parvalbumin gene: Detection and quantification by universal primers for forensic application

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10481057" target="_blank" >RIV/00216208:11310/24:10481057 - isvavai.cz</a>

Alternative codes found

RIV/00027022:_____/24:N0000034 RIV/60461373:22330/24:43928371

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=SdTubG~KGB" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=SdTubG~KGB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2024.106029" target="_blank" >10.1016/j.jfca.2024.106029</a>

Result language

angličtina

Original language name

Fish parvalbumin gene: Detection and quantification by universal primers for forensic application

Original language description

The substitution of illegal fish species poses economic, health and environmental risks. Identifying fish species, particularly closely related ones lacking external features, is a challenge. This study introduces an alternative approach using nuclear gene markers for positive detection, identification, and quantification of mitochondrial gene markers common in existing assays. We developed a universal primer targeting the parvalbumin (pvalb) beta gene, a common fish allergen with an amplicon size of 117 bp long. The developed fish-specific primer assay was tested on a diverse panel of 54 fish species, including commonly consumed species such as salmon, carp, and cod, along with non-fish species such as Buffalo, chicken and pork, and vegetables such as wheat, celery, and mint. The assay consistently identified the fish pvalb gene, demonstrating our assay&apos;s high specificity and effectiveness in detecting this gene in a variety of fish species. The assay exhibited a low limit of detection (LOD) of 5 pg, detecting trace DNA quantities. The assay also effectively quantified parvalbumin with good efficiency and linearity. These findings highlight pvalb as a reliable forensic tool for the identification of fish species. Implementing nuclear gene markers mitigates fish mislabelling and fraud, reducing economic and environmental impacts.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

<a href="/en/project/QK1910231" target="_blank" >QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

1096-0481

Volume of the periodical

128

Issue of the periodical within the volume

April

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

106029

UT code for WoS article

001187947800001

EID of the result in the Scopus database

2-s2.0-85185474560