A new approach for discriminating spatially acquired SERS spectra using antiretroviral drug emtricitabine as a test sample

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10490005" target="_blank" >RIV/00216208:11310/24:10490005 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Zaykfl_FSB" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Zaykfl_FSB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.6721" target="_blank" >10.1002/jrs.6721</a>

Result language

angličtina

Original language name

A new approach for discriminating spatially acquired SERS spectra using antiretroviral drug emtricitabine as a test sample

Original language description

In this study, an antiretroviral drug emtricitabine (also known as FTC or Emtriva) was detected and quantified down to 40 ng/ml by using surface-enhanced Raman spectroscopy (SERS). Its aqueous standards were tested with two types of silver nanoparticles (colloidal and dendritic) using a specially developed aluminum well plate. The SERS spectra were acquired using a Raman scanning device with 30-mu m spatial resolution. The spectral data were analyzed using a new approach for the discrimination of the spatially acquired spectra based on the Quality index (Qi). After the Qi is calculated, the spectral data are sorted based on the Qi. This is followed by selecting only the spectra with a high Qi index and comparing the average of all the spatially acquired spectra. This results in an improvement in the spectral signal-to-noise and higher analytical sensitivity of the built calibration curves. Spatially discrimination: improvement of the analytical sensitivity was accomplished by using spatial discrimination and comparing the averaging of all the acquired spectra to spatial discrimination followed by averaging. In this study, the quantification method for the antiretroviral drug emtricitabine (FTC or Emtriva (R)) at clinically relevant concentrations was reached. Two different types of silver nanoparticles were tested, colloidal and dendritic, and deposited on the aluminum well plate, which was placed in the automated Raman scanning device. image

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

1097-4555

Volume of the periodical

55

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1129-1138

UT code for WoS article

001279462000001

EID of the result in the Scopus database

2-s2.0-85200003858