A simplified system for the detection of antennal responses to host-borne volatile organic compounds in sand flies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10501333" target="_blank" >RIV/00216208:11310/25:10501333 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tOww-2ZM13" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tOww-2ZM13</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-025-06998-3" target="_blank" >10.1186/s13071-025-06998-3</a>
Alternative languages
Result language
angličtina
Original language name
A simplified system for the detection of antennal responses to host-borne volatile organic compounds in sand flies
Original language description
Phlebotomus (Larroussius) perniciosus (Diptera: Psychodidae) is the most common and predominant vector of Leishmania infantum in the Western Mediterranean region. Volatile organic compounds (VOCs) produced by vertebrates are important cues affecting the behaviour of blood-feeding insects. Generally, the identification of putative behaviourally active VOCs involves three distinct phases: extraction, chemical characterization and chemoreceptivity evaluation using electrophysiological techniques. Here, we present a simplified gas chromatography-mass spectrometry-electroantennographic detection (GC-MS-EAD) setup adapted for screening bioactive compounds in sand flies, in which the chemical identification and antennal responses are recorded simultaneously.The method integrates: (i) a flow-splitter that balances the flow rate of the two outgoing streams, (ii) GC columns with different lengths and diameters in the two sections splitter-MS and splitter-EAD and (iii) an antennal signal amplifier. The GC-MS-EAD analysis was applied to headspace solid-phase microextraction (HS-SPME) extracts from a healthy dog, and antennal responses were recorded in female P. perniciosus sand flies.The canine VOC profile was predominantly composed of aldehydes, with hexanal and nonanal eliciting the strongest antennal responses in P. perniciosus.This simplified GC-MS-EAD system shows promise for broader application in the study of host-vector interactions. Its use across different host-vector pairs may enhance our understanding of these relationships and inform the development of strategies for integrated vector monitoring and control.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10600 - Biological sciences
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasites & Vectors
ISSN
1756-3305
e-ISSN
1756-3305
Volume of the periodical
18
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
352
UT code for WoS article
001554048200002
EID of the result in the Scopus database
2-s2.0-105013582545