Electrochemical oxidation of non-aromatic sterols possessing double bond (s) in the steroid core on carbon-based electrodes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10504905" target="_blank" >RIV/00216208:11310/25:10504905 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JH9xM0bAIZ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JH9xM0bAIZ</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2025.146666" target="_blank" >10.1016/j.electacta.2025.146666</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical oxidation of non-aromatic sterols possessing double bond (s) in the steroid core on carbon-based electrodes
Original language description
Sterols are essential components of eukaryotic cell membranes. They possess a hydroxyl group at C(3) and an unsaturated steroid skeleton. Their detection in biological and physiological matrices is rather challenging, relying on MS techniques or enzymatic assays. This study focused on exploring the electrochemical behavior of sterols in dependence on positioning and number of double bonds within the steroid core using cyclic voltammetry. The experiments were performed in the media of perchlorate salt or perchloric acid in acetonitrile using glassy carbon and boron doped diamond electrode. Sterols featuring Δ(5) double bond (cholesterol, stigmasterol, β-sitosterol) and Δ(7) lathosterol provide primary oxidation signals around +1.5 V to +1.7 V (vs. Ag/AgNO3 in acetonitrile). The oxidation potential of Δ(8) lanosterol (ca +1.1 V) and Δ(5,7) sterols (ergosterol, 7-dehydrocholesterol, around +0.8 V to +1.0 V) is shifted to less positive potential values. Notably, in the presence of perchloric acid, the oxidation signals of Δ(5,7) sterols exhibit time instability. It can be attributed to structural changes induced by dehydration, similar to cholesterol dehydration in the Liebermann-Burchard reaction. The lowest detection limits were obtained by differential pulse voltammetry in 0.1 mol L(-1) HClO4 in acetonitrile and are in the range from 0.4 to 0.6 µmol L(-1) for Δ(5,7) and Δ(8) sterols on BDD electrode and from 1.1 to 6.1 µmol L(-1) for Δ(5) and Δ(7) sterols on GC electrode. This electrode has limited use for the detection of Δ(5) sterols with a highly positive oxidation potential, in the 0.1 mol L(-1) NaClO4 in acetonitrile media. This study extends the knowledge on sterol redox processes and shows the potential of simple electrochemical methods for sterol detection using unmodified electrode materials.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA19-11268S" target="_blank" >GA19-11268S: Electrochemical Methods: New Approaches for Characterization and Analysis of Steroids</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica Acta
ISSN
0013-4686
e-ISSN
1873-3859
Volume of the periodical
536
Issue of the periodical within the volume
October
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
146666
UT code for WoS article
001516777000001
EID of the result in the Scopus database
2-s2.0-105008335033