Surface plasmon resonance study on HIV-1 integrase strand transfer activity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F09%3A00206636" target="_blank" >RIV/00216208:11320/09:00206636 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/09:00332731 RIV/61388963:_____/09:00332731
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Surface plasmon resonance study on HIV-1 integrase strand transfer activity
Original language description
An assay for real-time label-free monitoring of the integrase (IN) activity based on surface plasmon resonance was developed. This assay enabled direct monitoring of the integration of a viral doubled-stranded DNA into the host genome. The strand transfer reaction was detected by using two different DNA targets: supercoiled plasmid (pUC 19) and short palindrome oligonucleotide. The effect of the length of the DNA target on the possibility to monitor the actual process of the strand transfer reaction isdiscussed. The assay developed can serve as an important analytical tool to search for potential strand transfer reaction inhibitors as well as for the study of compounds interfering with the binding of ds long terminal repeats-IN complexes with the hostDNA.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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