Effects of DPH on DPPC-Cholesterol Membranes with Varying Concentrations of Cholesterol: From Local Perturbations to Limitations in Fluorescence Anisotropy Experiments
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F10%3A10082407" target="_blank" >RIV/00216208:11320/10:10082407 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effects of DPH on DPPC-Cholesterol Membranes with Varying Concentrations of Cholesterol: From Local Perturbations to Limitations in Fluorescence Anisotropy Experiments
Original language description
We have used atomistic molecular dynamics simulations to consider 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescent probes in a fluid dipalmitoylphosphatidylcholine bilayer with 5 and 20 mol % cholesterol (CHOL). We show that while DPH affects a number ofmembrane properties, the perturbations induced by DPH depend on the concentration of cholesterol in a membrane. For example, we find DPH to influence the mass density distribution of lipids across the membrane and to promote the ordering of acyl chains around the probe. Yet, these perturbations get relatively weaker for increasing cholesterol concentration. Meanwhile, we also find that the commonly used analysis in terms of the Brownian rotational diffusion (BRD) model with Legendre polynomials to interpret fluorescence anisotropy (FA) experiments is sensitive to the amount of cholesterol. For small concentrations of cholesterol, the analysis of FA turns out to yield a relatively good approximation of the correct orientational distribut
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physical Chemistry B
ISSN
1520-6106
e-ISSN
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Volume of the periodical
114
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000274842600019
EID of the result in the Scopus database
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