SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F12%3A10126624" target="_blank" >RIV/00216208:11320/12:10126624 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1155/2012/393847" target="_blank" >http://dx.doi.org/10.1155/2012/393847</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2012/393847" target="_blank" >10.1155/2012/393847</a>

Result language
angličtina
Original language name
SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops
Original language description
We report the application of dried Ag hydroxylamine-reduced colloidal drops to surface-enhanced (resonance) Raman scattering (SE(R)RS) study of biomolecules using Raman microspectroscopy. 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (TMPyP), amino acid tryptophan, and phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) served as testing biomolecules. Ag colloid/biomolecule drop dried on glass support forms a ring in the edge part of the drop in which almost all nanoparticles are clustered. This specific drying process promotes adsorption of the studied biomolecules in highly enhancing sites ("hot spots") as well as concentrates them in the ring. We were able to obtain SE(R) RS spectra from the ring that cannot be acquired directly fromAg colloidal solutions (SERRS spectrum of 1 x 10(-10) M TMPyP by 1 s accumulation time, SERS spectrum of 2 x 10(-7) M DSPC). Despite the spectral irreproducibility and problems with spurious bands in some cases, SERS microspectroscopy of
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/GAP208%2F10%2F0941" target="_blank" >GAP208/10/0941: Engineering of surface-modified optical processes in molecules and semiconductor quantum dots by plasmon resonances in metal nanoparticle assemblies.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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