Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F12%3A10128431" target="_blank" >RIV/00216208:11320/12:10128431 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1155/2012/415496" target="_blank" >http://dx.doi.org/10.1155/2012/415496</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2012/415496" target="_blank" >10.1155/2012/415496</a>
Alternative languages
Result language
angličtina
Original language name
Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging
Original language description
Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into cancer MCF7 cells and the subsequent intracellular distribution. Phosphorothioate analog of 15-meroligoadenylate (dA(15)) labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H(2)TMPyP4) as an uptake-mediating agent. Fluorescence lifetime data within a broad spectral range have revealed properties of both components inside the cell. H(2)TMPyP4 lifetime inside the cell is not influenced in this malignant cell line, while the lifetime of modified oligonucleotide was found to be slightly shortened.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP208%2F10%2F0941" target="_blank" >GAP208/10/0941: Engineering of surface-modified optical processes in molecules and semiconductor quantum dots by plasmon resonances in metal nanoparticle assemblies.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
ISSN
0712-4813
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
5-6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
415-419
UT code for WoS article
000306505500020
EID of the result in the Scopus database
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