Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10286185" target="_blank" >RIV/00216208:11320/14:10286185 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c4pp00121d" target="_blank" >http://dx.doi.org/10.1039/c4pp00121d</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c4pp00121d" target="_blank" >10.1039/c4pp00121d</a>

Result language
angličtina
Original language name
Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells
Original language description
A new setup for direct microspectroscopic monitoring of singlet oxygen (O-1(2)) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect O-1(2). The O-1(2') phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of O-1(2) phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting n
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/GBP501%2F12%2FG055" target="_blank" >GBP501/12/G055: Photosynthesis Research Center</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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