5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10287787" target="_blank" >RIV/00216208:11320/14:10287787 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/14:00428075 RIV/61388963:_____/14:00428075
Result on the web
<a href="http://nar.oxfordjournals.org/content/42/8/5378.full?sid=eab1a866-369a-4bc8-8838-2585aa47d635" target="_blank" >http://nar.oxfordjournals.org/content/42/8/5378.full?sid=eab1a866-369a-4bc8-8838-2585aa47d635</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gku125" target="_blank" >10.1093/nar/gku125</a>

Result language
angličtina
Original language name
5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
Original language description
Several oligothymidylates containing various ratios of phosphodiester and isopolar 5'-hydroxyphosphonate, 5'-O-methylphosphonate and 3'-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5'-hydroxyphosphonate or 5'-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3'-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5'-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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