Distinguishing co-existing unimolecular and bimolecular structural forms using joint analysis of Raman and UV absorption data

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314373" target="_blank" >RIV/00216208:11320/15:10314373 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jrs.4610" target="_blank" >http://dx.doi.org/10.1002/jrs.4610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jrs.4610" target="_blank" >10.1002/jrs.4610</a>

Result language
angličtina
Original language name
Distinguishing co-existing unimolecular and bimolecular structural forms using joint analysis of Raman and UV absorption data
Original language description
Standard Raman measurements of solutions require relatively high concentration of dissolved species, which supports formation of intermolecular complexes. In case of simultaneous presence of unimolecular and bimolecular structural forms, it is often difficult or even impossible to discriminate between them by using only the Raman data. We present a possible and effective way how to combine temperature dependencies of Raman scattering and UV absorption in a joint thermodynamic analysis. This approach isbased on a joint fit of the results of multivariate analysis (singular value decomposition) of individual Raman and UV absorption spectral sets to a thermodynamic model. Our case study on RNA oligonucleotides, which can form imperfect intermolecular duplexes or intramolecular hairpin structures, demonstrates that this approach allows to determine thermodynamic parameters of co-existing unimolecular and bimolecular structural forms and to isolate their Raman spectra. Sons, Ltd.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—

Project
<a href="/en/project/GA202%2F09%2F0193" target="_blank" >GA202/09/0193: Formation and dynamics of nucleic acid motifs involved in regulation of gene expression</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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