The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314724" target="_blank" >RIV/00216208:11320/15:10314724 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c4pp00339j" target="_blank" >http://dx.doi.org/10.1039/c4pp00339j</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c4pp00339j" target="_blank" >10.1039/c4pp00339j</a>
Alternative languages
Result language
angličtina
Original language name
The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach
Original language description
The present work provides a proof-of-concept that the singlet oxygen-sensitized delayed fluorescence (SOSDF) can be detected from individual living mammalian cells in a time-resolved microscopy experiment. To this end, 3T3 mouse fibroblasts incubated with 100 mu M TPPS4 or TMPyP were used and the microsecond kinetics of the delayed fluorescence (DF) were recorded. The analysis revealed that SOSDF is the major component of the overall DF signal. The microscopy approach enables precise control of experimental conditions - the DF kinetics are clearly influenced by the presence of the O-1(2) quencher (sodium azide), H2O/D2O exchange, and the oxygen concentration. Analysis of SOSDF kinetics, which was reconstructed as a difference DF kinetics between the unquenched and the NaN3-quenched samples, provides a cellular O-1(2) lifetime of tau(Delta) = 1-2 mu s and a TPPS4 triplet lifetime of tau(T) = 22 +/- 5 mu s in agreement with previously published values. The short SOSDF acquisition times,
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP501%2F12%2FG055" target="_blank" >GBP501/12/G055: Photosynthesis Research Center</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photochemical and Photobiological Sciences
ISSN
1474-905X
e-ISSN
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Volume of the periodical
14
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
700-713
UT code for WoS article
000352283100007
EID of the result in the Scopus database
2-s2.0-84926469106