Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10316952" target="_blank" >RIV/00216208:11320/15:10316952 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1155/2015/842810" target="_blank" >http://dx.doi.org/10.1155/2015/842810</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2015/842810" target="_blank" >10.1155/2015/842810</a>
Alternative languages
Result language
angličtina
Original language name
Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif
Original language description
Effect of cytosine methylation on DNA duplexes was studied by using a model system of three self-complementary DNA octamers containing central CpG motif surrounded by a couple of AT base pairs, CAACGTTG, CATCGATG, and CTTCGAAG, and their analogues with the central cytosine methylated at C5 position. Temperature dependences of H-1 NMR, UV absorption, and Raman scattering spectra measured for aqueous solutions at concentrations of different orders of magnitude were subjected to a joint analysis that allowed an accurate determination of the enthalpy and entropy of duplex formation. It was revealed that the changes of the enthalpy and entropy contributions are strongly dependent on the base composition in the vicinity of the CpG motif.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-26526S" target="_blank" >GA13-26526S: Novel DNA and RNA oligonucleotides with Phosphonothioate and Phosphonoamidate Internucleotide Linkages.</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Spectroscopy
ISSN
2314-4920
e-ISSN
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Volume of the periodical
2015
Issue of the periodical within the volume
2015
Country of publishing house
EG - EGYPT
Number of pages
8
Pages from-to
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UT code for WoS article
000360755300001
EID of the result in the Scopus database
2-s2.0-84941126144