Effect of ribose versus 2 '-deoxyribose residue in guanosine 5 '-monophosphates on formation of G-quartets stabilized by potassium and sodium cations

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F16%3A10332426" target="_blank" >RIV/00216208:11320/16:10332426 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.vibspec.2015.12.002" target="_blank" >http://dx.doi.org/10.1016/j.vibspec.2015.12.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vibspec.2015.12.002" target="_blank" >10.1016/j.vibspec.2015.12.002</a>

Result language
angličtina
Original language name
Effect of ribose versus 2 '-deoxyribose residue in guanosine 5 '-monophosphates on formation of G-quartets stabilized by potassium and sodium cations
Original language description
Raman spectroscopy was used to directly compare different abilities of monomeric ribo- and 2'- deoxyribo guanosine 5'-monophosphates to constitute G-quartets and their supramolecular selfassemblies stabilized by Na+ and K+ cations in pH-neutral or slightly basic aqueous solutions. Nucleotide concentration, stoichiometry and temperature was systematically varied to show that in the presence of Na+ cations, 5'-dGMP remains unordered under the conditions when 5'-rGMP already forms Na+-stabilized G-quartets and soluble ordered assemblies. On the contrary, in the presence of K+ ions both 5'-GMPs readily form self-stacking G-quartets, although self-association of 5'-rGMP is still greater than that of 5'-dGMP. These results are supported by 1H and 31P NMR spectroscopy. The cation-sensitive effect of ribose versus 2'-deoxyribose substitution on the formation of G-quartets and soluble aggregates from monomeric 5'-guanosine monophosphates under physiological pH could be essential for full understanding of different structural polymorphism of RNA and DNA quadruplexes.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/GA15-06785S" target="_blank" >GA15-06785S: Nucleic Acid Regulatory Sequences - polymorphism, dynamics, and interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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