Origin of enhanced VCD in amyloid fibril spectra: Effect of deuteriation and pH
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F17%3A10361856" target="_blank" >RIV/00216208:11320/17:10361856 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/chir.22722" target="_blank" >http://dx.doi.org/10.1002/chir.22722</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chir.22722" target="_blank" >10.1002/chir.22722</a>
Alternative languages
Result language
angličtina
Original language name
Origin of enhanced VCD in amyloid fibril spectra: Effect of deuteriation and pH
Original language description
Supramolecular chirality of amyloid fibrils, protein aggregates related to many neurodegenerative diseases, is a remarkable property associated with fibril structure and polymorphism. Since its discovery almost 10 years ago there is still little understanding of this phenomenon, including the cause of the highly enhanced vibrational circular dichroism (VCD) intensity arising from fibril supramolecular chirality. In this study, VCD spectra, enhanced by filament supramolecular chirality, are presented for lysozyme and insulin fibrils above and below pH 2 and after deuterium exchange, above and below pD 2. Supramolecular chirality (observed by VCD) and fibril morphology (documented by atomic force microscopy) are not affected by protein deuteriation. In D2O the fibril VCD sign pattern changes to fewer bands, with implications for the amide I/II origin of enhanced VCD intensity. Separation of amide I and II signals will facilitate calculations of enhanced VCD spectra of amyloid fibrils and enable a better understanding of the origin of the VCD sign pattern.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chirality
ISSN
0899-0042
e-ISSN
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Volume of the periodical
2017
Issue of the periodical within the volume
29
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
469-475
UT code for WoS article
000409526100001
EID of the result in the Scopus database
2-s2.0-85023628441