Gold film over very small (107nm) spheres as efficient substrate for sensitive and reproducible surface-enhanced Raman scattering (SERS) detection of biologically important molecules

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F18%3A10386423" target="_blank" >RIV/00216208:11320/18:10386423 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21340/18:00319912

Result on the web

<a href="https://doi.org/10.1002/jrs.5317" target="_blank" >https://doi.org/10.1002/jrs.5317</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.5317" target="_blank" >10.1002/jrs.5317</a>

Result language

angličtina

Original language name

Gold film over very small (107nm) spheres as efficient substrate for sensitive and reproducible surface-enhanced Raman scattering (SERS) detection of biologically important molecules

Original language description

Gold plasmonic nanostructures with high sensitivity and spectral reproducibility are key components of molecular sensors based on surface-enhanced Raman scattering (SERS). In this paper, we report a bottom-up fabrication of gold film over nanosphere (FON) substrates for SERS sensing on the basis of very small (107nm in diameter) polystyrene spheres coated with 20nm of gold. To obtain close-packed spheres in a large scale area, the self-assembly at the water-air interface was found to be very efficient. Sensitivity and reproducibility of the AuFON substrates were tested using various molecular probes: 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin, p-aminothiophenol, and benzocaine. Relative standard deviation of SERS signal was found to be less than 20% confirming good spectral reproducibility especially using a 100x objective. Then, we demonstrated sensitive SERS detection of other biologically important molecules adsorbed on our AuFON substrates: thiolated-polyA, protoporphyrin IX, and two alkaloids (nicotine and strychnine). Estimated limits of detection were 8.2x10(-7) and 4.5x10(-7)M (in order of 100ng.ml(-1)) incubation concentration for strychnine and nicotine, respectively. Therefore, we conclude that the 20-nm gold film deposited on polystyrene spheres of 107nm in diameter provided both good spectral reproducibility and sensitivity. Their implementation into the SERS-active system will enable to bring about new quantitative and analytical SERS applications.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/GA13-20110S" target="_blank" >GA13-20110S: New substrates and approaches for sensitive and reproducible biomolecular sensing by means of surface-enhanced Raman scattering (SERS) spectroscopy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

—

Volume of the periodical

49

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

499-505

UT code for WoS article

000426512700012

EID of the result in the Scopus database

2-s2.0-85042859063