The effect of Chelidonius majus alkaloids on tumor and yeast cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00003448" target="_blank" >RIV/00216224:14110/00:00003448 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
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Original language name
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
Original language description
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and
Czech name
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Czech description
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Project
<a href="/en/project/GA204%2F99%2FD025" target="_blank" >GA204/99/D025: The role of cytoskeleton and cell wall in the cell polarization</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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