Detection of spectrin-like protein in yeasts: spectrin-actin relations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F02%3A00006732" target="_blank" >RIV/00216224:14110/02:00006732 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of spectrin-like protein in yeasts: spectrin-actin relations

Original language description

Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa inS. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electr

Czech name

Detection of spectrin-like protein in yeasts: spectrin-actin relations

Czech description

Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa inS. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electr

Type

D - Article in proceedings

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník X. Cytoskeletálního klubu

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

14

Publisher name

Československá biologická společnost

Place of publication

Praha

Event location

Vranovská Ves

Event date

Apr 24, 2002

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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