Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F03%3A00008710" target="_blank" >RIV/00216224:14110/03:00008710 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/03:00007991 RIV/00216224:14110/03:00008552

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.

Original language description

Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions. Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers. As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells werecultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-[alpha], Flt-3, CD40L, IFN-[gamma], IL-1[alpha], IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Vaccine

ISSN

0264-410X

e-ISSN

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Volume of the periodical

neuveden

Issue of the periodical within the volume

2/2003

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

877-882

UT code for WoS article

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EID of the result in the Scopus database

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