Comparative genomics of pathogenic Treponema pallidum subspecies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F03%3A00012312" target="_blank" >RIV/00216224:14110/03:00012312 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparative genomics of pathogenic Treponema pallidum subspecies
Original language description
DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. Noregion of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Czech name
Komparativní genomika patogenních poddruhů Treponema pallidum
Czech description
Byly vyšetřeny kmeny Treponema pallidum subsp. pallidum (původce syfilis) a T. pallidum subsp. pertenue (původce yaws) pomocí DNA-microarray techniky a genomového sekvenování. Zjištěné rozdíly byly diskutovány v kontextu současných poznatků.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NI7351" target="_blank" >NI7351: Comparative genomics of pathogenic spirochetes of the genus Treponema: the route towards sequence-specific diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CHEMICKÉ LISTY
ISSN
0009-2770
e-ISSN
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Volume of the periodical
2003
Issue of the periodical within the volume
97
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
2
Pages from-to
297-298
UT code for WoS article
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EID of the result in the Scopus database
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