Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F05%3A00012467" target="_blank" >RIV/00216224:14110/05:00012467 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
Original language description
C. laurentii is occasionally diagnosed as an opportunistic infectious agent in immuno- suppressed patients, in the last years (1). The cytoskeleton of this yeast has not been studied yet. We examined the distribution and the dynamics of F-actin and microtubules in two strains of the yeast C. laurentii - encapsulated and non-encapsulated. The visualization of actin cytoskeleton by means of rhodamine-phalloidin in encapsulated strain C. laurentii CCY 17-3-5 failed. When observed, only the weak diffusionalfluorescence in the cells was identified. However, we detected the actin patches in the mother cells and their intensive fluorescence in the growing buds, which occured after application of monoclonal anti-actin antibody and consecutive secondary antibody SwAM-FITC. The second strain C. laurentii 17-3-6, which does not form capsule, was relatively more accessible for finding of F-actin using rhodamine-phalloidin. The cortical actin patches were detected.
Czech name
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Czech description
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Project
<a href="/en/project/GA310%2F03%2F1195" target="_blank" >GA310/03/1195: The cytoskeleton as the target structure for the study of the effect of antifungal agents on pathogenic yeasts</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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