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Vinca alkaloid effect on microtubules of HeLa cells.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00016405" target="_blank" >RIV/00216224:14110/06:00016405 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Vinca alkaloid effect on microtubules of HeLa cells.

  • Original language description

    The study evaluated the effect of selected vinca alkaloids on the microtubule network of heLa cells and the recovery of its disruption. Model experiments were used for demonstration of the quantification of microtubule network changes induced by vinca alkaloids using computer-assisted image analysis. Differences in the arrangement of microtubules were assessed by means of a software for quantification of the cytoskeleton changes in cells treated with vincristine at a concentration of 0,020 g/l and in untreated control cells. The microtubule network recovery process in time intervals of 6, 7, 8, 9, and 10 hours was quantified after treatment with vincristine at a concentration of 0,020 g/l for 60 min using the software for quantification of the cytoskeleton changes.

  • Czech name

    Účine vinca alkaloidů na mikrotubuly HeLa buněk.

  • Czech description

    heLa buňky byly ovlivněny vinkristinem nebo vinblastinem o koncentraci od 0,002 do 0,8 g/l po dobu 60ti minut nebo vinkristinem o koncentraci 4,615 g/l a 9,230 g/l po dobu 60ti minut. Mikrotubuly byly detekovány metodou nepřímé imunofluorescence a poškození bylo stanoveno pomocí fluorescenčního mikroskopu. Změny v uspořádání mikrotubulů byly u buněk ovlivněných vinkristinem o koncentraci 0,020 g/l po dobu 60 min a u buněk neovlivněných hodnoceny pomocí softwaru pro kvantifikaci cytoskeletálních změn. Dále byl s použitím softwaru pro kvantifikaci změn cytoskeletu studován proces reparace mikrotubulů v časových intervalech 6, 7, 8, 9 a 10 hodin, po ovlivnění vinkristinem o koncentraci 0,020 g/l po dobu 60 minut.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GD301%2F03%2FH005" target="_blank" >GD301/03/H005: Molecular and cell biology in biomedicine</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scripta Medica

  • ISSN

    1211-3395

  • e-ISSN

  • Volume of the periodical

    79

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    14

  • Pages from-to

    19-34

  • UT code for WoS article

  • EID of the result in the Scopus database