Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00018909" target="_blank" >RIV/00216224:14110/06:00018909 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

Original language description

Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes wastested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)?based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitroto numbers useful for clinical applications.

Czech name

Izolace a expanze alogenních myelom-specifických a interferon gama-pozitivních T lymfocytů pro adoptivní imunoterapii

Czech description

Izolace a expanze alogenních myelom-specifických interferon-gama produkujících T lymfocytů pro buněčnou imunoterapii u mnohočetného myelomu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NC7475" target="_blank" >NC7475: Evaluation of Cellular Immunity and Molecular Analysis of Myeloma-Specific T cells in Patients with Multiple Myeloma</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Oncology

ISSN

1559-131X

e-ISSN

—

Volume of the periodical

23/2006

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

—

UT code for WoS article

000242136000009

EID of the result in the Scopus database

—