Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00029526" target="_blank" >RIV/00216224:14110/09:00029526 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS
Original language description
Myocardial ischemia and reperfusion phenomena have been studied repeatedly in various models. The aim of this study was to verify preconditioning model in guinea pig and rabbit Langendorff-perfused hearts loaded with voltage-sensitive dye (VSD) di-4-ANEPPS which we use to record monophasic action potentials (MAPs) by optical method. The heart was perfused according to Langendorff at constant pressure (80mmHg) with Krebs Henseleit solution (KH, 37C, 1.25mM Ca2+). Each experiment consisted of heart isolation, control perfusion, dye loading, washout and MAPs recording. In our previous experiments, we have observed and consequently studied specific electrophysiological changes during the procedure of VSD loading and washout in both species. These changes were more expressed in guinea pig myocardium, thus we started to use rabbit hearts for most of our experiments with VSD.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů