Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00048691" target="_blank" >RIV/00216224:14110/10:00048691 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of Cytoskeleton Inhibitors on Conidiogenesis and Capsule in the Long Neck Yeast Fellomyces Examined by Scanning Electron Microscopy

Original language description

The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. During conidiogenesis,mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. Conclusion: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/GA310%2F06%2F0605" target="_blank" >GA310/06/0605: Human pathogenic fungi : the cytoskeleton as a potential target of inhibition of morphogenesis.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemotherapy

ISSN

0009-3157

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

6

Pages from-to

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UT code for WoS article

00278957700005

EID of the result in the Scopus database

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