Sample Processing and Methodological Pitfalls in Multiple Myeloma Research

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00049835" target="_blank" >RIV/00216224:14110/11:00049835 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/11:#0001306

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sample Processing and Methodological Pitfalls in Multiple Myeloma Research

Original language description

In this paper, initial processing of biological material, cell separation algorithms and other procedures are discussed. For samples with initial infiltration of plasma cells &gt; 5%, CD138 MicroBeads and Auto-Magnetic-Activated Cell Sorting program areused. Fluorescence-Activated Cell Sorting is used exclusively for cell populations with low-abundance; these samples are detected using fluorescently labeled antibodies only. Isolated plasma cells are further processed for molecular biological studies, for cytogenetics and protein analyses. Furthermore, this work examines the pitfalls of research related to multiple myeloma; some of them we have overcome, while the others are still problematic.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Klinická onkologie

ISSN

0862-495X

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

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Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

18-23

UT code for WoS article

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EID of the result in the Scopus database

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