Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00050081" target="_blank" >RIV/00216224:14110/11:00050081 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/jmicro/dfr073" target="_blank" >http://dx.doi.org/10.1093/jmicro/dfr073</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jmicro/dfr073" target="_blank" >10.1093/jmicro/dfr073</a>

Result language

angličtina

Original language name

Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast

Original language description

Temperature-sensitive actin mutant of Saccharomyces cerevisiae act1-1 was studied at a permissive temperature of 23 C by light, fluorescent and electron microscopy to elucidate the roles of actin cytoskeleton in the cycling eukaryotic cells. Mutant cellsthat grew slowly at the permissive temperature showed aberrations in the cytoskeleton and cell cycle. Mutant cells contained aberrant faint actin cables, that failed in directing of mitochondria, vacuoles and secretory vesicles to the bud and the strayvesicles delivered their content to the mother wall instead of the bud. Bud growth was delayed. Spindle pole bodies and cytoplasmic microtubules did not direct to the bud, and nucleus failed to migrate to the bud. Repeated nuclear divisions produced multinucleated cells, indicating continued cycling of actin mutant cells that failed in the morphogenetic checkpoint, the spindle position checkpoint and cytokinesis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA310%2F06%2F0605" target="_blank" >GA310/06/0605: Human pathogenic fungi : the cytoskeleton as a potential target of inhibition of morphogenesis.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Electron Microscopy

ISSN

0022-0744

e-ISSN

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Volume of the periodical

60

Issue of the periodical within the volume

6

Country of publishing house

JP - JAPAN

Number of pages

13

Pages from-to

379-391

UT code for WoS article

000297867800005

EID of the result in the Scopus database

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