Human embryonic stem cells suffer from centrosomal amplification
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00051844" target="_blank" >RIV/00216224:14110/11:00051844 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/11:00367964
Result on the web
<a href="http://dx.doi.org/10.1002/stem.549" target="_blank" >http://dx.doi.org/10.1002/stem.549</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/stem.549" target="_blank" >10.1002/stem.549</a>
Alternative languages
Result language
angličtina
Original language name
Human embryonic stem cells suffer from centrosomal amplification
Original language description
Propagation of human embryonic stem cells (hESCs) in culture tends to alter karyotype, potentially limiting the prospective use of these cells in patients. The chromosomal instability of some malignancies is considered to be driven, at least in part, bycentrosomal overamplification, perturbing balanced chromosome segregation. Here, we report, for the first time, that very high percentage of cultured hESCs has supernumerary centrosomes during mitosis. Supernumerary centrosomes were strictly associated with an undifferentiated hESC state and progressively disappeared on prolonged propagation in culture. Improved attachment to culture substratum and inhibition of CDK2 and Aurora A (key regulators of centrosomal metabolism) diminished the frequency of multicentrosomal mitoses. Thus, both attenuated cell attachment and deregulation of machinery controlling centrosome number contribute to centrosomal overamplification in hESCs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cells
ISSN
1066-5099
e-ISSN
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Volume of the periodical
29
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
46-56
UT code for WoS article
000286659300007
EID of the result in the Scopus database
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