Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00056991" target="_blank" >RIV/00216224:14110/11:00056991 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201100430" target="_blank" >10.1002/elps.201100430</a>

Result language

angličtina

Original language name

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients

Original language description

Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-20-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/NS10200" target="_blank" >NS10200: Tumor markers, their determination and correlation with prostate carcinoma</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

22

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

3207-3220

UT code for WoS article

000298098700018

EID of the result in the Scopus database

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