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The relationship between MSX1 gene mutation and tooth agenesis in the Czech population

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00065637" target="_blank" >RIV/00216224:14110/13:00065637 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S0901502713010278" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0901502713010278</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ijom.2013.07.734" target="_blank" >10.1016/j.ijom.2013.07.734</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The relationship between MSX1 gene mutation and tooth agenesis in the Czech population

  • Original language description

    Tooth development (odontogenesis) is a very complicated and complex process involving interplay between oral epithelium and adjacent ectomesenchyme. These reciprocal interactions are mediated by more than 300 signaling molecules (e.g. PAX9, AXIN2 and MSX1). Mutations in the genes for these two regulation proteins were associated with isolated (non-syndromic) form of hypodontia and for this reason; we decided to study gene mutations for MSX1 gene. Upon receipt of approval from the Ethical Commission of University Hospital Ostrava (No. 638/2011), DNA was isolated from patients using Ultra Clean Blood Spin DNA Isolation Kit (Mo-Bio). Blood samples or buccal swabs from 200 Czech patients with various types of tooth agenesis were used for this purpose. PCRproducts for exons of MSX1 gene were prepared using Kapa 2G Robust Hot Start polymerase (KapaBio Systems) and sequenced using ABI 3130 Prism (Life Technologies) genetic analyzer.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NT11420" target="_blank" >NT11420: Molecular diagnostics of hypodontia and possibilities of tooth autotransplantation</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů