Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00076848" target="_blank" >RIV/00216224:14110/14:00076848 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors
Original language description
Minimal residual disease (MRD) detection allowed acquisition of valuable prognostic information in several mature lymphoid disorders with a considerable impact on clinical research (Ferrero S. 2011, Pott C. 2010). Although different methods can be used for MRD quantification, including flow cytometry (FC), real-time quantitative polymerase chain reaction (qPCR) remains the most validated and standardized method (Ladetto M. 2013, Pott C. 2011). However, despite remarkable sensitivity and specificity, qPCR has some drawbacks mainly related to the need for a reference standard curve, performed by target serial dilutions. The introduction of droplet digital PCR (ddPCR) has allowed to overcome this limitation, since its absolute quantification competence allows to avoid the need for a reference standard curve.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů