A high-throughput siRNA screen identifies genes that regulate mannose 6-phosphate receptor trafficking
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00078588" target="_blank" >RIV/00216224:14110/14:00078588 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1242/jcs.159608" target="_blank" >http://dx.doi.org/10.1242/jcs.159608</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jcs.159608" target="_blank" >10.1242/jcs.159608</a>
Alternative languages
Result language
angličtina
Original language name
A high-throughput siRNA screen identifies genes that regulate mannose 6-phosphate receptor trafficking
Original language description
The delivery of newly synthesized soluble lysosomal hydrolases to the endosomal system is essential for lysosome function and cell homeostasis. This process relies on the proper trafficking of the mannose 6-phosphate receptors (MPRs) between the trans-Golgi network (TGN), endosomes and the plasma membrane. Many transmembrane proteins regulating diverse biological processes ranging from virus production to the development of multicellular organisms also use these pathways. To explore how cell signaling modulates MPR trafficking, we used high-throughput RNA interference (RNAi) to target the human kinome and phosphatome. Using high-content image analysis, we identified 127 kinases and phosphatases belonging to different signaling networks that regulate MPR trafficking and/or the dynamic states of the subcellular compartments encountered by the MPRs. Our analysis maps the MPR trafficking pathways based on enzymes regulating phosphatidylinositol phosphate metabolism.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cell Science
ISSN
0021-9533
e-ISSN
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Volume of the periodical
127
Issue of the periodical within the volume
23
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
5079-5092
UT code for WoS article
000345896800009
EID of the result in the Scopus database
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