Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00078632" target="_blank" >RIV/00216224:14110/14:00078632 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1128/AEM.01619-14" target="_blank" >http://dx.doi.org/10.1128/AEM.01619-14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.01619-14" target="_blank" >10.1128/AEM.01619-14</a>

Result language

angličtina

Original language name

Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance

Original language description

Leptospira spp. are spirochete bacteria comprising both pathogenic and free-living species. The saprophyte L. biflexa is a model bacterium for studying leptospiral biology due to relative ease of culturing and genetic manipulation. In this study, we constructed a library of 4,996 random transposon mutants in L. biflexa. We screened the library for increased susceptibility to the DNA intercalating agent, ethidium bromide (EtBr), in order to identify genetic determinants that reduce L. biflexa susceptibility to antimicrobial agents. By phenotypic screening, using subinhibitory EtBr concentrations, we identified 29 genes that, when disrupted via transposon insertion, led to increased sensitivity of the bacteria to EtBr. At the functional level, these genes could be categorized by function as follows: regulation and signaling (n = 11), transport (n = 6), membrane structure (n = 5), stress response (n = 2), DNA damage repair (n = 1), and other processes (n = 3), while 1 gene had no predicte

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

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Volume of the periodical

80

Issue of the periodical within the volume

19

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

6091-6103

UT code for WoS article

000341887100024

EID of the result in the Scopus database

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