Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084662" target="_blank" >RIV/00216224:14110/15:00084662 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1099/mic.0.000091" target="_blank" >http://dx.doi.org/10.1099/mic.0.000091</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/mic.0.000091" target="_blank" >10.1099/mic.0.000091</a>
Alternative languages
Result language
angličtina
Original language name
Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae
Original language description
Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Burker Chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance ofactin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing beta-1,3-glucan microfibrils. The LA-inhibited cells lysed.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbiology-SGM
ISSN
1350-0872
e-ISSN
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Volume of the periodical
161
Issue of the periodical within the volume
"Part 7"
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
1348-1355
UT code for WoS article
000361411900006
EID of the result in the Scopus database
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