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Label-free and amplification-free miR-124 detection in human cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00085315" target="_blank" >RIV/00216224:14110/15:00085315 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/15:43907632 RIV/00216305:26620/15:PU118479

  • Result on the web

    <a href="http://dx.doi.org/10.3892/ijo.2014.2756" target="_blank" >http://dx.doi.org/10.3892/ijo.2014.2756</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3892/ijo.2014.2756" target="_blank" >10.3892/ijo.2014.2756</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Label-free and amplification-free miR-124 detection in human cells

  • Original language description

    MicroRNAs (miRNAs) are becoming a very important group of molecules especially since their connection to numerous diseases has been revealed. The potential in gene therapy as well as in diagnostics is being widely investigated leading to the demand of sensitive, selective and simple methods of isolation and detection. The combined advantages of magnetic particle-based separation with sensitive electrochemical detection may offer a very valuable tool for these purposes. In this study, the miR-124 was targeted as an example analyte for development and optimization of the isolation procedure coupled to the electrochemical detection. The sensitivity of the method was demonstrated by the limit of detection at the level of nanomolar concentration (4 nM).

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP102%2F11%2F1068" target="_blank" >GAP102/11/1068: Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal of Oncology

  • ISSN

    1019-6439

  • e-ISSN

  • Volume of the periodical

    46

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    GR - GREECE

  • Number of pages

    7

  • Pages from-to

    871-877

  • UT code for WoS article

    000347671200049

  • EID of the result in the Scopus database