Rapid detection of fungal pathogens in bronchoalveolar lavage samples using panfungal PCR combined with high resolution melting analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00087628" target="_blank" >RIV/00216224:14110/16:00087628 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/16:00065722
Result on the web
<a href="http://dx.doi.org/10.1093/mmy/myw032" target="_blank" >http://dx.doi.org/10.1093/mmy/myw032</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/mmy/myw032" target="_blank" >10.1093/mmy/myw032</a>
Alternative languages
Result language
angličtina
Original language name
Rapid detection of fungal pathogens in bronchoalveolar lavage samples using panfungal PCR combined with high resolution melting analysis
Original language description
Despite advances in the treatment of invasive fungal diseases (IFD), mortality rates remain high. Moreover, due to the expanding spectrum of causative agents, fast and accurate pathogen identification is necessary. We designed a panfungal polymerase chain reaction (PCR), which targets the highly variable ITS2 region of rDNA genes and uses high resolution melting analysis (HRM) for subsequent species identification. The sensitivity and specificity of this method was tested on a broad spectrum of the most clinically important fungal pathogens including Aspergillus spp., Candida spp. and mucormycetes. Despite the fact that fluid from bronchoalveolar lavage (BAL) is one of the most frequently tested materials there is a lack of literature sources aimed at panfungal PCR as an IFD diagnostic tool from BAL samples. The applicability of this method in routine practice was evaluated on 104 BAL samples from immunocompromised patients.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/TE02000058" target="_blank" >TE02000058: Center of competence for molecular diagnostics and personalized medicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Medical Mycology
ISSN
1369-3786
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
714-724
UT code for WoS article
000384204100006
EID of the result in the Scopus database
2-s2.0-84991208366